牛A型巴氏杆菌高、低毒力株LPS激活RAW264.7细胞TLR4信号通路的比较分析

doi:10.11843/j.issn.0366-6964.2019.03.017

Abstract

Abstract:

This study was designed to investigate the effects of TLR4 signaling pathway in RAW264.7 cells treated with lipopolysaccharide (LPS) in two different virulent Pasteurella multocida serotype A isolates (high-virulent PmCQ2 and low-virulent PmCQ6) respectively. The activation of TLR4 pathway, expression of TNF-α and IL-12p40, phosphorylation of IκBα, and nuclear translocation of NF-κB, were identified in LPS-stimulated RAW264.7 cells. Results showed that the expression of TLR4, TNF-α and IL-12p40 was significantly increased in LPS-stimulated RAW264.7 cells. Furthermore, both LPS_PmCQ2 and LPS_PmCQ6 up-regulated IκBα phosphorylation, and activated TLR4-triggered nuclear translocation of NF-κBp65, but there was no significant difference between the two treatments. The LPS of bovine Pasteurella multocida Serotype A high-and low-virulent strains can induce the TLR4-mediated immune response, and there is no difference in TLR4-mediated IκBα-NF-κB signaling pathway, indicating that there may be no significant difference in virulence between LPS_PmCQ2 and LPS_PmCQ6 on RAW264.7 cells. These results suggested that for RAW264.7 cells, the virulence of Pasteuralla multocida is dependent on others virulence factors, not LPS.

CLC Number:

S852.612

LI Yanhong, LIU Jie, LI Weiping, CUI Yuting, PENG Yuanyi, WU Zhengli. Comparative Analysis of TLR4 Signaling Pathway in RAW264.7 Cells Activated by LPS from High-and Low-virulence Strains of Bovine Pasteurella multocida Serotype A[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 620-626.

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Comparative Analysis of TLR4 Signaling Pathway in RAW264.7 Cells Activated by LPS from High-and Low-virulence Strains of Bovine Pasteurella multocida Serotype A

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